University of California, San Diego - BENG 16809_10

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BENG 168 – Biomolecular Engineering Spring Quarter, 2016 Midterm Study Guide *The exam starts at 5:00 pm and end at 6:20pm on Thursday Feb, 4th. Closed book, closed notes. No calculators, phones... , etc. The following topics should be important and clearly understood: 1. DNA, RNA, Proteins: features of building blocks, replication, transcription, translation, posttranslational modifications, particularly phosphorylation and glycosylation 2. DNA technologies: 1. cut/paste, restriction enzymes, ligases, 2. amplification, PCR, 3. Measure DNA, electrophoresis, hybridization, southern/northern, microarray, FISH, quantitative PCR, 3. DNA cloning: vector, origin of replication, multiple cloning site, promoter, terminator, stop codon, antibiotic resistant gene, selection marker 4. DNA synthesis: synthesis cycle, phosphoramidites, coupling efficiency/accuracy, DNA assembly strategies 5. DNA sequencing: Sanger method, pyrosequencing, primer walking 6. Genome sequencing: shotgun cloning sequencing, cyclic array sequencing 7. Genetic engineering and proteomics, 2D electrophoresis, Mass spectrum analysis, protein arrays, recombinant cloning 8. Gene manipulation in prokaryotes: promoter and promoter regulation, fusion proteins, immunotherapy, features of translational expression vectors, optimization strategies of protein production in prokaryotes, DNA integration into chromosomes, protein secretion 9. Gene manipulation in eukaryotes: 1. Fungus (s. cerevisiae), 2. Baculovirus-insect cells, 3. mammalian cells; general features of translational expression vectors for eukaryotes, DNA integration into chromosomes, humanization, engineering eukaryote cells, the effect of epigenetic and chromatin structures.2.4 Double-stranded DNA [Show More]

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