BIOS242-11983 Microbiology LAB Final Study guide.

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BIOS242-11983 Microbiology LAB Final Study guide. 1. When aseptically inoculating media, why do you sterilize the wire loop? To sterilize the loop as to not introduce microbes to the sample. -To pre... vent the spreading of microbes from one culture to the next. 2. When performing the 4-quadrant streak method, how do you reduce the number of bacteria on the wire loop? Pass wire loop on the burner flame and let it cool after streak and picking up some bacteria at every streak 3. What is the best way to store bacterial samples? Refrigerator 4. What are the required steps to ASEPTICALLY inoculate sterile culture tubes in order to prevent possible contamination? Why do you do these steps? Open at a 45-degree angle Flaming mouth of tube To prevent contamination 5. Know the meanings of the safety hazard pictograms for oxidizing reagents, flammable, health hazard, explosive and corrosive materials. How should you dispose of hazardous material generated in a lab? Acute Toxicity Aquatic Toxicity (fatal or toxic) Environment (Non-Mandatory) Skull and Crossbones Oxidizers Explodong bomb Flame Over Circle Self-Reactives Organic Peroxides Explosives Corrosion Gas Cylinder Skin Corrosion/Burns Gases Under Pressure • Eye Damage • Corrosive to Metals Exclamation Mark Flame Irritant (skin and eye) Flammables • Skin Sensitizer Pyrophorics • Acute Toxicity (harmful) Self-Heating • Narcotic Effects Emits Flammable Gas • Hazardous to Ozone Self-Reactives Layer (Non-Mandatory) Organic Peroxides Respiratory Tract Irritant Health Hazard [Show More]

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