Clinical Chemistry > LECTURE NOTES > Liver Function Test detailed notes (All)
USED TO Detect presence of liver disease Distinguish among different types of liver ds Gauge the extent of known liver damage Follow the response of treatment R... arely suggest a specific diagnosis functions Serum bilirubin Urine bilirubin Blood ammonia Serum enzymes : AST, ALT, GGT, 5’Nucleotidase,ALP of liver Serum Albumin Serum Globulins PT ,INR A break down product of porphyrin ring of heme – containing proteins , found in blood in 2 fractions – conj/unconj Conjugated : water soluble , so excreted by kidneys Unconjugated : insoluble in water , bound to albumin in blood About 300 mg of bilirubin is formed per day Normal total serum bilirubin: 0.3 – 1.3 mg/dl Direct/conjugated bilirubin: 0.1 – 0.4 mg/dl Indirect/unconjugated bilirubin: 0.2 – 0.9mg/dl Measured by Van Den Bergh method Bilirubin reacts with diazo reagent to produce coloured azo pigment . At pH 5 – pigment purple . [Show More]
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