THE EFFECT OF CAFFEINE ON THE HEART RATE OF DAPHNIA

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THE EFFECT OF CAFFEINE ON THE HEART RATE OF DAPHNIA HYPOTHESIS: As the caffeine concentration rises, so does the mean heart rate of daphnia. RISK ASSESSMENT: In this experiment there are the foll... owing risks both for the human that carries out the experiment and the daphnia:  The human that carries out the experiment should be cautious not to touch the lamp with wet hands since it uses electricity.  Also, be careful not to spill any of the pond water on the floor.  As for the daphnia, there is a chance for them to die as when they are exposed to light, they are also exposed to heat in which they aren’t used to.  Moreover, when they are given caffeine of a high concentration their heart may beat so fast that will cause their death.  Furthermore, there is the risk of the daphnia to dry up, which may kill it in the middle of the experiment. METHOD: These are the steps that we followed when doing the experiment: 1. 1. Use a pipette to transfer the daphnia to a cavity slide containing pond water of known volume. 2. 2. Add some cotton fibres to the cavity slide and move them with a fine paint brush in such a way that the daphnia can’t move. 3. 3. Use a calculator to calculate the heart rate. Set its reading to zero and add 1 to the answer [Show More]

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