Multi column Purification of rGFP University of Texas, Dallas - BIOCHEM 3380LAB 5.

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Experiment 5 – Lab Report Multi column Purification of rGFP To save time, simply edit this word document by inserting your answers after each question/space. Please denote your answers by using b... old, italics, or colored font. Do NOT renumber/reorganize the questions. “Showing your work/calculations” can be achieved by typing it out, pasting in a clear image of your handwritten work, or using handwritten annotation on the document (if you have the technology to do so). Insert graphs (handwritten on graph paper or electronically constructed with horizontal/vertical gridlines) as a clear image with appropriate titles, labeled/scaled axes, and standard deviation bars when appropriate. 1. My assigned data set for experiment 4 was:________ 16 (1pt) Which two fractions did you pool from column 1 (Ni+2 agarose) to apply to column 2 (anion exchanger)? The highest fluorescence activity in elution and wash fractions was E2 (15,955RFU) and E3 (12,515RFU) respectively. 2. My assigned data set for experiment 4 was:_______ (3pts) What was the amount of rGFP activity that you loaded onto column 2? Show your calculations for credit. E2 (15,955RFU) and E3 (12,515RFU) 6382/200ul =xRFU/500ul 15,955 5006/200ul =xRFU/500ul 12,515 3. (3pts) Based upon the protocol, predict a reasonable void volume for the anion exchanger? Explain your reasoning for full credit [Show More]

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