QUESTIONS AND ANSWERS FOR BIOL 204 FINAL EXAM-ALL ANSWES CORRECT-GRADED A

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BIOL  204  – Biochemistry Name:   SPRING  2017/REA/ELLIOTT/KARCH Penn  ID  (middle  8  digits):   1. The  five  proteins,  listed  in  the ...  table  below  with  their  estimated  Mr and  pI  values,  were   separated  by  two-­‐dimensional  electrophoresis. Protein Mr pI a. Unknown 45,000 5.4 b. Cytochrome  c 13,400 10.6 c. Myoglobin 17,000 7.0 d. Serum  albumin 69,000 4.8 e. Transferrin 90,000 5.9 A.   Indicate  the likely  positions of  the  proteins  listed  in  the  table on  the  Coomassie   blue-­‐stained  gel  shown  below: 2.5 pts B. Assuming  that  all  of  the  proteins  in  part  A are  monomeric and  have  similar   hydrodynamic  diameters,  in  what  order  would  you  expect  them  to  elute  from  a   gel-­‐filtration  column  when  it  is  run  under  non-­‐denaturing  conditions? 1 pt A. b, c,  a,  d,  e B. e,  d,  a,  c,  b C. d,  a,  e,  c,  b D. b,  c,  a,  e,  d E. b,  c,  e,  a,  d a b c d e3 BIOL  204  – Biochemistry Name:   SPRING  2017/REA/ELLIOTT/KARCH Penn  ID  (middle  8  digits):   C. In  what  order  would  you  expect  the  proteins  in  part  A to  desorb  from  a  strong   cation-­‐exchange  resin  eluted  with  a  linear  gradient  of  NaCl? 2.5 pts A. b,  c,  a,  d,  e B. e,  d,  a,  c, b C. d,  a,  e,  c,  b D. b,  c,  a,  e,  d E. b,  c,  e,  a,  d 2. [Show More]

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