BioChem PA

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 PCR o Polymerase Chain Reaction o What do we need for PCR  Target DNA  dNTPs = deoxnucleotides  DNA Primers  TAQ Polymerase = Enzyme “Ase”  Stable at high temperatures o... Steps for PCR  1. Denaturation  Sample is heated to 95 degrees C to separate the 2 DNA strands  2. Annealing = “Sticks”  Next, cooled to 50 degrees C. Primers bind (base pair) to the template DNA  3. Elongation  DNA Polymerase works well at 70 degrees C- DNA Polymerase synthesizes new strands of DNA starting at the primers  Typical there will be about 30 rounds of PCR  2n = # DNA molecules produces o 2-1 = 2 o 2-2 =4 [Show More]

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