MB (ASCP) 2022/2023 Exam Questions and Answers (Complete Solution) Graded A+

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MB (ASCP) 2022/2023 Exam Questions and Answers (Complete Solution) Graded A+ Consider the table below where a child and a possible father (PF) share the listed paternity indices for each locus lis... ted (LOC-A1, LOC-B2, LOC-C3, LOC-D4). Locus Tested PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 15/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table, what is the combined paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4? 12.42 9.558 15.56 2.38 ✔✔ 12.42 ( CPI is calculated by multiplying all Paternity index tgt) Best way to avoid PCR contamination ✔✔ Separate Pre-PCR (clean area) and post PCR (dirty area) A260=1.4, A280=1.1 . protein contamination or Rna contam or useable RNA ✔✔ Protein contamination . A260/280 = 1.3 <1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-2.0 = good RNA Why is Fluorometry a better technique that Spectroscopy ✔✔ Increased sensitivity and more importantly can accurately measure DNA concentration of a structurally intact double stranded DNA molecule Linkage Disequilibrium ✔✔ LD is the non random association of alleles at 2 or more loci in a general population. When alleles are in LD, haplotypes do not occur at the expected frequencies. Specificity ✔✔ Specificity = TN / (TN + FP) Limit of Detection (LOD) ✔✔ lowest concentration of analyte that can be detected mRNA Sequence provided - Convert it into DNA ✔✔ (Template/Anti-sense Strand) [Replace U with T] Correct order of HIV Sample Preparation Workflow ✔✔ Plasma Preparation RNA Extraction RNA Amplification/Modification Procedure If a flask of cells is provided for the intended purpose of DNA isolation, how would you proceed further ✔✔ Trypsinize and/or scrape the cells inside the provided flask, collect the cells suspended in the media, spin, and then proceed further Which end of the primer is critical for the increased specification of PCR Amplification process ✔✔ 3'End of the Primer Primer Dimer Complementarity ✔✔ 5' End of the Primers (3-3' Was not an option) Mechanism of Action of MecA Gene in MRSA ✔✔ Altered Drug Target (PBP2A) Technique to be used for FFPE Cervical Tissue Provided ✔✔ Invader Assay (Non-Amplification Method) Liquid Based-Cytology Useful for what kind of virus ✔✔ HPV is the correct answer (Options Provided for: HIV, HBV, HCV, HPV) Isothermal Method that utilizes Primes and Polymerase to Create Synthetic dsDNA ✔✔ SDA (Strand Displacement Amplification) Polymerase used in Isothermal LAMP Procedure ✔✔ BST (Bacillus Stearothermophilus) BRAF V600E Mutation is Associated with ✔✔ Lynch Syndrome (Also Known as HNPCC) CAP Requires Discontinued SOP/Procedures to be stored for how long ✔✔ At least 2 years from the date of discontinuation CAP Requires Laboratory Personnel (Technologist) Proficiency Testing ✔✔ Once a Year Most Important Component of Clinical Laboratory Completed Validation Studies ✔✔ Final Test Validation Summary Signed off by Laboratory Director STR Electropherogram provided and select the most informative loci ✔✔ Two different alleles each was both recipient vs donor the most informative one STR makes use of which of the following ✔✔ Variable Nucleotide Experiment Performed (RFLP + PCR + Gel Electrophoresis): three bands observed in the mutant sequence as compared to the normal sequence ✔✔ Insertion of New Restriction Site [Show More]

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