Restriction endonuclease - -cleaves DNA molecules at a specific sequence of bases à molecular scissors, Laboratory tools, wide spectrum of application. They have sticky and blunt ends. -Enzymes Us... ed in DNA Technology - -I. Restriction endonuclease -cleaves DNA molecules at a specific sequence of bases II. DNA ligase -joins DNA strands together III. DNA polymerase -makes DNA from nucleotides -Taq DNA polymerase IV. Reverse transcriptase -generates complementary DNA (cDNA) from an RNA template -Restriction Fragment Length Polymorphism (RFLP) - -•A technique that exploits variations in DNA sequences. -Variations include DNA length & Mutation. -Restriction sites recognized by restriction endonucleases generate DNA fragments with varied length -Almost obsolete, replaced by DNA sequencing -molecular cloning - -isolation and incorporation of a piece of DNA into a vector so it can be replicated and manipulated. -Applications include: Gene expression, Production of recombinant proteins, & gene therapy [Show More]
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Restriction endonuclease - -cleaves DNA molecules at a specific sequence of bases à molecular scissors, Laboratory tools, wide spectrum of application. They have sticky and blunt ends. -Enzymes Use...
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acid - -In the context of cell biology, an organic molecule that dissociates in water to generate hydronium (H3O+) ions (thereby producing a low pH). It donates a protein to a water moelcule (dissocia...
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