PORTAGE LEARNING BIOD 171 LAB EXAMS 1-8 LECTURE EXAMS 2022

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PORTAGE LEARNING BIOD 171 LAB EXAMS 1-8 LECTURE EXAMS 2022 Lecture Exams Answer the following questions. 1. At what temperature is the fixed incubator set to, as presented in the lab video? 37°C 2. A... t what temperature should you refrigerate bacterial samples? Explain why this is beneficial. 4°C. This temperature slows bacterial growth and prolongs the life of the sample. 3. What are the FOUR types of gloves presented in the lab video? Latex, Nitrile, Thermal cold, Thermal heat 4. What THREE rules were discussed in regards to lab safety that would protect you and others from contamination? 1. Never eat or drink in the lab 2. Always wear appropriate PPE 3. Never leave the lab wearing PPE 5. What are the main sections that should be found in a lab notebook? Name at least 4. Objective, Procedure, Notes, Results and Deviations Answer the following questions. 1. You are a lab instructor and Paul Smith has turned in his lab notebook for you to grade the lab experiment #2 on microscopy. Based upon what was covered in the lab video, how should Paul have entitled his lab experiment? PS02 Microscopy 2. You arrive to your first day of work at a new lab. You are taking over for someone who took a new job at another lab. Your boss informs you that because of time restraints, this person did not exactly follow the experimental protocol. In order to proceed, you must know what he did differently. (1) According to the lab lecture, under what section of his notebook would you look to find the experimental steps? (2) As changes to the experimental steps were made, what are these differences called and how should it appear in the lab notebook? (1) Procedure—this is where the steps for the experimental protocol are recorded. (2) Deviations. All deviations should be written in red to immediately bring attention to the changes in the protocol. Answer the following questions 1. Identify the part of the microscope indicated by the arrow. Neck (or arm) 2. Identify the part of the microscope indicated by the arrow. Stage 3. Identify the part of the microscope indicated by the arrow. fine focus Answer the following questions 1. You are about to study a bacterial sample under a light microscope. You look into the oculars and see two circles. What adjustments need to be made? Compress or expand the oculars until a single circle can be seen while using both eyes simultaneously. 2. What 2 parts of the microscope contributes to the total magnification to your sample? Objective and oculars (eyepiece) 3. As you looking through the microscope you wish to dim (or limit) the amount of light entering into the eyepiece—what component of the microscope other than the light source itself can be adjusted to make these modifications? Diaphragm [Show More]

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