BioChemistry > EXAM > ACS BIOCHEMISTRY EXAM – OBU Latest Graded A+ (All)
Separation of Organelles / Fractionation of Cells - ANSWER Differential Centrifugation 1. Tissue Homogenization 2. Low speed centrifugation (whole cells, nuclei, membrane) 3. Medium speed centrifu... gation (mitochondria, lysosomes, peroxisomes) 4. High speed centrifugation (microsomes -fragments of ER) 5. Very high-speed centrifugation (soluble proteins) Isopycnic Centrifugation - ANSWER Sucrose-Density (components separated by density) Interactions occur... - ANSWER because of the electrostatic properties of molecules. - these properties can involve full, partial or momentary charges Examples of electrostatic interactions - ANSWER dipole-dipole interactions hydrogen bonds ionic interactions van der Waals interactions Hydrophobic Interactions... - ANSWER entropy, not an attraction between hydrophobic molecules The special properties of water... - ANSWER derived from its polarity and hydrogen bonding - overall polarity is due to polar bonds and shape Entropy & 2 Critical Processes that involve Water - ANSWER 1. Dissolving of highly ordered solids (salts) - entropy of some of the water is decreased, but the entropy of the ions is greatly increased 2. The hydrophobic effect - there is no strong attraction between hydrophobic molecules (van der Waal's only) - there is no repulsion between hydrophobic molecules and water Aggregation of H-bonds in water is driven by - ANSWER entropy change of water (decrease) - it can also drive other reactions such as enzyme-substrate interaction Ion Product Constant - ANSWER [H+][OH-] = Keq [HOH] = 1.0 x 10^-14 Equilibrium Constant - ANSWER Keq = [H+][OH-]/[HOH] 2 Applications of pH - ANSWER 1. Controlling pH with buffers 2. How pH (protonation) affects weak interactions of molecules (charge and ability to form H-bonds) pKa = - ANSWER -log Ka pKa definition - ANSWER pH at which half of the acid is ionized. titration curves reveal the pKa of weak acids - is a measure of affinity for protons a high pKa constitutes... - ANSWER it takes a high pH (lots of OH-) to remove the H+ from the acid - the acid has a high affinity for H+ - if the pKa is high, it has a HIGH affinity (want) to grab onto H+, because of the large amount of OH- Significance of Buffering - ANSWER 1. Homeostasis 2. This phenomenon is very important for biochemical activity - many reactions depend on the state of protonation of particular functional groups A buffer is made... - ANSWER from a weak acid and its conjugate base Henderson-Hasselbalch Equation - ANSWER pH = pKa + log ([A-]/[HA]) [Show More]
Last updated: 1 year ago
Preview 1 out of 9 pages
Instant download
Buy this document to get the full access instantly
Instant Download Access after purchase
Add to cartInstant download
Biochemistry ACS Final Exam latest updated 2022 ACS BIOCHEMISTRY EXAM – OBU Latest Graded A+ ACS BIOCHEMISTRY EXAM Q&A With complete solution
By MARKALLAN 1 year ago
$15.5
3
Connected school, study & course
About the document
Uploaded On
Aug 24, 2022
Number of pages
9
Written in
This document has been written for:
Uploaded
Aug 24, 2022
Downloads
0
Views
45
In Browsegrades, a student can earn by offering help to other student. Students can help other students with materials by upploading their notes and earn money.
We're available through e-mail, Twitter, Facebook, and live chat.
FAQ
Questions? Leave a message!
Copyright © Browsegrades · High quality services·