BIOS 390 Week 6 LAB: Isolate Plasmid DNA (GRADED)

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Once a plasmid has … introduced into competent bacterial cells and the cells have grown into colonies on a medium selective for cells containing the plasmid, the next step is to perform a miniprep o... f the plasmid DNA in preparation for DNA sequencing. The three steps are: 1) growing cells in liquid culture; 2) purifying the plasmid DNA from the culture; and 3) performing a restriction digest on the purified DNA to determine whether the DNA insert in the plasmid is the … size. To start the liquid culture, cells from an isolated bacterial colony are … in selective medium (nutrient broth with an antibiotic to which the plasmid provides resistance). ……….. document continue [Show More]

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